A guide to methods in the biomedical sciences /
| Main Author: | |
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| Format: | Book |
| Language: | English |
| Published: |
New York :
Springer,
2004.
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| Subjects: | |
| Online Access: | http://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=130157 |
Table of Contents:
- Cover
- Acknowledgements
- Table of Contents
- Chaper 1 DETECTION AND ANALYSIS OF PROTEINS
- A. Introduction
- B. Basic methods for protein analysis
- C. Characterization of primary, secondary, tertiary
- Chaper 2 DETECTION AND ANALYSIS OF NUCLEIC ACIDS
- A. Introduction
- B. Basic methods for nucleic acid analysis
- C. DNA sequencing
- D. Detection and quantitation of RNA
- Chaper 3 RECOMBINANT DNA TECHNIQUES: CLONING AND MANIPULATION OF DNA
- A. Introduction
- B. Plasmid and viral vectors
- C. Libraries
- D. Site Directed Mutagenesis
- E. Polymerase chain reaction (PCR)
- F. Screening for differentially expressed genes (DEGs)
- G. Promoter-protein interactions
- H. Silencing gene expression
- I. Forensics and DNA technology
- Chaper 4 ANTIBODY-BASED TECHNIQUES
- A. Introduction
- B. Monoclonal antibodies
- C. Purification and use of antibodies
- D. Flow cytometry and fluorescence activated cell sorting (FACS)
- E. Other assays using antibodies
- Chaper 5 MICROSCOPY: IMAGING OF BIOLOGICAL SPECIMENS
- A. Introduction
- B. Light microscopy
- C. Fluorescence and laser confocal microarray microscopy
- D. Electron Microscopy (EM)
- E Magnetic resonance imaging (MRI)
- Chaper 6 THE DERIVATION AND MANIPULATION OF EXPERIMENTAL ANIMALS IN BIOMEDICAL SCIENCES
- A. Introduction
- B. Inbred strains of mice
- C. Congenic strains of mice
- D. Transgenic mice
- E. Targeted transgenes: "knockout" and "knockin" mice
- F. Other uses of mice in biomedical research
- References
- Some excellent methodology manuals.